Fig. 7

Differential metabolites induced by ICA treatment. a Volcano map of metabolites for groups (CON vs. LCD and ICA2 vs. LCD) in positive and negative iron modes. b The shared and unique numbers of differential metabolites in the Venn diagram from CON vs. LCD and ICA2 vs. LCD. c Heat map and hierarchical clustering of 8 shared differential metabolites among the CON, LCD and ICA2 groups. ICA, icariin. CON, base diet. LCD, low calcium diet. ICA2, low calcium diet supplied with 2.0 g/kg ICA