Fig. 3
From: Identification and phylogenetic analysis of two canine coronavirus strains

Growth kinetics of CCoV JS1706 and CCoV JS1712 in A-72 cells. A-72 cells were infected with JS1706 and JS1712 at an MOI (multiplicity of infection) of 0.05. Infected cells and culture supernatant were harvested every 12 h for 72 h. Virus titer was determined by TCID50 (50% tissue culture infective dose). Data are representative of three independent experiments performed in triplicate and presented as the means ± SD