Fig. 4

Immunoblots of liver microsomes and serum from various species with anti-rat Ces1d. Microsomes (10 μg) or serum (0.5 μL) from mature males were resolved by 7.5% SDS-PAGE and transferred electrophoretically to nitrocellulose membranes. The blots were blocked by milk and detected by the antibody against recombinant rat Ces1d through E. coli expression system. This antibody has been shown to have a broad-cross reactivity activity among carboxylesterases