Fig. 3

Isolation and genotyping of Brucella from sheep blood cell lysates. A Identification of Brucella isolates by BCSP31 gene PCR amplification. M, 2,000Â bp DNA ladder. Lane 1, B. melitensis M5-90 strain. Lane 2, B. melitensis TZ strain. Lane 3, E. coli ATCC25922 strain. B Identification of Brucella isolates by OMP 25 gene PCR amplification. M, 2000Â bp DNA ladder. Lane 1, B. melitensis M5-90 strain. Lane 2, B. melitensis TZ strain. Lane 3, E. coli ATCC25922 strain. C Identification of Brucella species by AMOS typing. M, Â 2000Â bp DNA ladder. Lane 1, B. melitensis M5-90 strain. Lane 2, B. abortus A19 strain. Lane 3, B. suis S2 strain. Lane 4, B. melitensis TZ strain