Table 3 Primers used in the present study
Primers | Sequences (5′-3′) | Product size (bp) | Tm (°C) | Description | References |
|---|---|---|---|---|---|
Bacterial species identification genes | |||||
 Fla1 | TGGCGCCTGCCCTATC | 237 | 56 | Bb identification | Hozbor et al. 1999 |
 Fla2 | AGGCTCCCAAGAGAGAAA |  |  | ||
Virulence factors encoding genes | |||||
 FhaB-1 | GCGCAGAACATCACCAATG | 475 | 59 | Filamentous hemagglutinin encoding gene | Wang et al. 2020 |
 FhaB-2 | TGAAATACTCCATGGCGGAC | ||||
 Prn-1 | GACCTCGCTCAGTCGATC | 555 | 59 | Pertactin encoding gene |  |
 Prn-2 | GAAGACATTCATGCGGAACAG |  |  |  |  |
 CyaA-1 | CTACGAGCAGTTCGAGTTTC | 377 | 59 | Adenylate cyclase-hemolysin toxin encoding gene |  |
 CyaA-2 | TATTCATGTCGCCGTCGTA |  |  |  | |
 Dnt-1 | TGATCCTGCAGTGGTTGATC | 491 | 59 | Dermonecrotic toxin encoding gene |  |
 Dnt-2 | ATCGGCATACGCCAGATC |  |  |  | |
 BteA-1 | TGTTGAGCAACAACGTCAATC | 474 | 59 | Bordetella type-III secretion system effector A encoding gene |  |
 BteA-2 | TATGCAGGTCTTCGAGGTTC |  |  | ||