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Table 2 Repeatability of cELISA

From: Preparation of a monoclonal antibody against recombinant LSDV034 protein and its application in detecting lumpy skin disease virus through a competitive enzyme-linked immunosorbent assay (cELISA)

Reference serum

Repeatability (OD450 values)

Coefficient of variation

(CV %)

Test 1

Test 2

Test 3

1 ( +)

0.507

0.463

0.502

4.910

2 ( +)

0.482

0.516

0.444

7.493

3 ( +)

0.377

0.340

0.341

5.977

4 (-)

1.819

1.853

1.780

2.010

5 (-)

1.110

0.964

1.003

7.370

6 (-)

0.955

1.034

0.993

3.975

  1. " + " indicates that the serum antibody was positive; "-" indicates that the serum antibody was negative