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Fig. 3 | Animal Diseases

Fig. 3

From: Novel triplex nucleic acid lateral flow immunoassay for rapid detection of Nipah virus, Middle East respiratory syndrome coronavirus and Reston ebolavirus

Fig. 3

Optimization of 5’ primer label/tag and antibody combinations for NALFIA. A Hexaplex format; T6 - Dinitrophenyl (DNP), T5 - Digoxigenin (DIG), T4 - Fluorescein, T3 - Texas red, T2 - Alexa fluor 488, T1 - Rhodamine. Of the 6 label and antibody combinations tested, DNP and anti-DNP showed no reactivity, while the Texas Red antibody showed cross-reactivity with multiple labels. B Triplex format; three labels, DIG (T3), rhodamine red (T2) and Alexa fluor 488 (T1), were finally selected, and all further experiments were conducted using this combination

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Animal Diseases

ISSN: 2731-0442