Fig. 7

A 272 bp gene construct composed of NiV N (100 bp), REBOV Vp40 (80 bp) and MERS-CoV UpE (92 bp) synthesized in tandem at the EcoRI site in the pBluescript II SK (+) vector (vector sequence is not included in the figure). The NCBI GenBank accession numbers of the sources of the target genes are EU620498.1 (NiV), AB050936.1 (REBOV), and JX869059.2 (MERS-CoV). These selected gene target regions are highly conserved, and the details of the sequence alignment results can be found in Additional file 2