Table 3 Hapten labels for 5’ end-labeling of forward and reverse primers and their corresponding antibodies
Combination | 5’ hapten label on forward primer | Antibody against 5’ label on forward primer | 5’ hapten label on reverse primer |
|---|---|---|---|
1 | Dinitrophenyl (DNP), IDT | Polyclonal anti-DNP ab, molecular probe | Biotin, Eurofins |
2 | Digoxigenin (DIG), Eurofins | Polyclonal anti-DIG ab, pierce | Biotin, Eurofins |
3 | Texas Red, Eurofins | Polyclonal anti-texas red ab, molecular probe | Biotin, Eurofins |
4 | Fluorescein, Eurofins | Polyclonal anti-fluorescein ab, molecular probe and monoclonal anti-fluorescein ab, molecular probe | Biotin, Eurofins |
5 | Rhodamine Red, Eurofins, IDT | Polyclonal anti-tetramethyl rhodamine ab, molecular probe | Biotin, Eurofins |
6 | Alexa fluor 488, Eurofins | Polyclonal anti-alexafluor 488 ab, molecular probe | Biotin, Eurofins |