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Table 4 Layout of hexaplex and triplex NALFIA strips with specific order of the label antibodies as test lines

From: Novel triplex nucleic acid lateral flow immunoassay for rapid detection of Nipah virus, Middle East respiratory syndrome coronavirus and Reston ebolavirus

Hexaplex

Triplex

 

Absorbtion Pad

Absorbtion pad

 

Control line

Conjugate control

Conjugate control

Control line

Test line 1

Anti-DNP ab

Anti-DIG ab

Test line 1

Test line 2

Anti-DIG ab

Anti- Rhodamine ab

Test line 2

Test line 3

Anti-Fluorescein ab

Anti-Alexa Fluor 488 ab

Test line 3

Test line 4

Anti-Texas Red ab

Sample pad/Conjugate pad

 

Test line 5

Anti-Alexa fluor 488 ab

Test line 6

Anti-Rhodamine ab

 

Sample pad/Conjugate pad

  1. DNP Dinitrophenyl, DIG Digoxigenin, ab Antibody