Table 5 Simulated positive samples used for triplex NALFIA evaluation
Species | Sample type | IVT RNA | No. of Samples spiked | Amount of IVT RNA per extraction | RNA extraction by |
|---|---|---|---|---|---|
Bat (Pteropus) | Oro-pharyngeal swab | NiV N | 2 |  ~ 28 pg | TRIzol LS |
REBOV Vp40 | 2 |  ~ 5.5 pg | |||
MERS UpE | 3 |  ~ 230 fg | |||
Pig | NiV N | 2 |  ~ 28 pg | ||
REBOV Vp40 | 1 |  ~ 5.5 pg | |||
Camel | Serum | MERS UpE | 3 |  ~ 230 fg | |
Pig | NiV N | 1 |  ~ 28 pg | ||
REBOV Vp40 | 1 |  ~ 5.5 pg | |||
Camel | Tissue | MERS UpE | 6 |  ~ 460 fg | TRIzol |
Pig | NiV N | 3 |  ~ 56 pg | ||
REBOV Vp40 | 3 |  ~ 11 pg | |||
Bat (Pteropus) | Whole blood | NiV N | 3 |  ~ 28 pg | |
REBOV Vp40 | 3 |  ~ 5.5 pg |