Fig. 7
The effects of ASFV MGF genes on cell innate immune responses. A 293 T cells in 96-well plate were transfected with pcGAS-HA (10 ng/well), pSTING-HA (10 ng/well), ISRE-Fluc or NF-κB-Fluc (10 ng/well) and Rluc (0.2 ng/well), together with MGF110-7L-2HA, Myc-MGF110-13L, Myc-MGF360-2L and Myc-MGF360-13L (10 ng and 20 ng each MGF plasmid) for 24 h. The promoter activity was measured as described in the Methods and the expressions of different proteins were examined by WB with relevant antibodies including anti-HA, anti-Myc and anti-β-Actin mAbs. B PAMs in 12-well plates were infected with three ASFVs (MOI = 0.1) for 96 h. The cells were examined for the mRNA expressions of IFN-β, ISG56, ISG15, TNF-α, IL-6 and IL-8 by RT‒qPCR assay. The three ASFV replications were monitored by using the WB detection of viral p72 expression