Fig. 2

Sensitivity test of SYBR Green I fluorescent PCR. Note that 1 × 107 to 0.1 copies/µL standard plasmids were used as templates for pMD-18 T-ASFV I (A: orange lines; B) and pMD-18 T-ASFV II (C: purple lines; D) detection. M: DNA Marker 2000; 1–9:107–0.1 copies/µL standard template; + : positive control; NC: nuclease-free water