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Table 1 Repeatability evaluation of the SYBR Green I fluorescent PCR for intra-assay and interassay variation

From: Development and application of a SYBR Green I fluorescent PCR assay for the differentiation of genotypes I and II African swine fever viruses

Dilution

(copies/µL)

pMD-18 T-ASFV I

pMD-18 T-ASFV II

Intra-assay variationa

Interassay variationb

Intra-assay variationa

Interassay variationb

(Ct)c

CV (%)

(Ct)c

CV (%)

(Ct)c

CV (%)

(Ct)c

CV (%)

1

19.14 ± 0.05

0.25

19.00 ± 0.26

0.85

18.97 ± 0.06

0.32

18.04 ± 0.13

0.74

2

26.69 ± 0.19

0.71

28.54 ± 0.90

0.90

27.32 ± 0.11

0.39

28.77 ± 0.25

0.86

3

34.87 ± 0.33

0.94

34.33 ± 0.39

1.13

33.60 ± 0.30

0.90

32.62 ± 0.30

0.93

  1. CV coefficient of variation
  2. aIntra-assay variation was determined on three replicates of recombinant plasmid dilutions analyzed in the same PCR run
  3. bInterassay variation was calculated on the basis of the results from three independent PCR runs
  4. cMean ± standard deviation (SD)
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Animal Diseases

ISSN: 2731-0442