Fig. 5

Molecular docking and immunohistochemistry results. A Three-dimensional binding of small molecules to target proteins and two-dimensional interactions are shown in the figure, and hydrogen bonding between small molecules and target proteins is shown in cyan. B Immunohistochemistry of Wenchang chicken lung tissue. The nuclei are shown in blue, and the brown areas indicate positive protein expression. The arrows indicate representative positive areas. The darker the brown color is, the higher the protein expression in that region. n=6. Three pathological sections were prepared for each chicken, and six fields of view were randomly selected from each section to capture images. 200× scale bar: 50 μm, one-way ANOVA, ****: p < 0.0001; **: p < 0.01. C Results of H&E staining of heart, liver, spleen, lung and kidney tissues from female and male rats, n=10. Three pathological sections were prepared for each rat, and six fields of view were randomly selected to capture images for each section. Scale bars are 200 μm. Heart, liver, lung and kidney tissues were imaged at 200× magnification, and spleen tissues were imaged at 100× magnification