Fig. 3

Muscle-specific knockout of G9a alleviates muscular atrophy in aged mice. A Body weights of young (left) and aged (right) WT and Ehmt2^Ckmm KO mice. B Quantification of the skeletal muscle weight/body weight ratio in aged WT and Ehmt2^Ckmm-KO mice. C Representative images of H&E staining (left), average (middle) and distribution (right) of myofiber CSA in the TAs of aged WT and Ehmt2^Ckmm KO mice. D qPCR results of Ehmt2 and muscular atrophy genes (Trim63 and Fbxo32) in the TAs of young and aged WT and Ehmt2^Ckmm KO mice. E Western blots (up) with quantitative results (bottom) of G9a and muscular atrophy markers (MuRF1 and Atrogin-1) in the TAs of young and aged WT and Ehmt2^Ckmm-KO mice. Gastroc, gastrocnemius; TA, tibialis anterior; EDL, extensor digitorum longus; CSA, cross-sectional area; n = 3–8 per group; ns, no significant difference, *P < 0.05, **P < 0.01