Fig. 1

Analysis of the monoclonal antibodies (mAbs) against PDCoV. (A) Antibody binding to PDCoV-infected LLC-PK1 cells, labeled with 1C2 or 5H5. Magnification, × 100. (B) mAb reactivity with PDCoV-infected LLC-PK1 cells assessed by flow cytometry. Red: PDCoV-infected cells with 1C2; green: PDCoV-infected cells with 5H5; black: PDCoV-infected cells with only secondary antibody; blue/yellow: LLC-PK1 cells with 1C2/5H5 as negative controls. (C) ELISA evaluated mAb reactivity to PDCoV. Negative serum was control. Measured at 450 nm