Fig. 1

Inhibitory effects of nucleoside analogs on PEDV infection. A The structure of nucleoside analogs. B The antiviral effects of nucleoside analogs were evaluated via indirect immunofluorescence. Microscope scale: 100 μm. IFA images showing the viral N protein in green and nuclei in blue. The experiments were performed three times and from 1 random horizon in each experiment. C Vero cells were infected with PEDV at 0.1 MOI in the presence of the nucleoside analogs at 20 μM for 18 h, and the virus titer was measured via a TCID₅₀ assay. The data were expressed as the means and standard deviations from three independent experiments and were analyzed via one-way ANOVA. ns, p > 0.05; ***, p < 0.001