Fig. 4

Gemcitabine synergizes with baicalein to inhibit PEDV proliferation. A A CCK-8 assay was performed to detect the viability of cells treated with different concentrations of the combination of gemcitabine and baicalein. B TCID₅₀ assay of the effects of the combination of gemcitabine and baicalein on the PEDV titer. C Evaluation of the effects of the combination of gemcitabine and baicalein on PEDV. D ZIP model evaluation of the combined effect of baicalein and gemcitabine. The synergetic volume regions are highlighted in red. The mean synergy score for a drug combination was averaged over all the dose combination measurements. E Effects of the combination of gemcitabine and baicalein on PEDV N protein expression, as measured by IFA. Green light represents the expression of the PEDV N protein