Fig. 3

Screening and identification of nanobodies (Nbs) against PEDV-S. A Detection of the periplasmic extracts from the specific clones that bind to PEDV-S via indirect ELISA with PEDV-S as the coating antigen. B Amino acid alignment of 10 screened (Nbs). The residues at positions 37, 44, 45 and 47 are indicated by red arrows, and hydrophilic amino acids are features of (Nbs). C Titers of the 10 (Nbs) in the periplasmic extracts that bind to PEDV-S