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Table 2 Optimized amount of PEDV-S protein and dilution of PEDV-S-Nb13-HRP fusion protein

From: A competitive ELISA based on nanobodies for the detection of serum neutralizing antibodies against porcine epidemic diarrhea virus

PEDV-S protein (ng/well)

Dilutions of PEDV-S-Nb13-HRP

1:1

1:10

1:100

1:200

80

1.99

1.852

1.272

1.012

100

2.286

2.213

1.512

1.111

200

2.456

1.952

1.585

1.344

400

2.123

2.203

1.799

1.346