A competitive ELISA based on nanobodies for the detection of serum neutralizing antibodies against porcine epidemic diarrhea virus

Table 4 Optimized incubation and color reaction time of the pig sera and PEDV-S-Nb13-HRP fusion protein

Times of color reaction (min)	Sera type	Incubation times of pig sera, ASFV-Nb75-HRP fusions and coated antigens (min)
Times of color reaction (min)	Sera type	30	40	60
10	Positive	0.589	0.650	0.418
	Negative	1.300	1.489	1.226
	P/N	0.453	0.437	0.341
15	Positive	0.554	0.594	0.220
	Negative	1.300	1.489	1.226
	P/N	0.426	0.399	0.179