Fig. 5

Schematic representation and evaluation of the specificity and sensitivity of the indirect competitive enzyme-linked aptamer-sorbent assay (ic-ELASA). A Schematic of the ic-ELASA method. PRV competes with HRP-streptavidin for binding to biotinylated aptamers, enabling PRV detection. B Evaluation of the specificityof ic-ELASA for detecting purified PRV, PEDV, and PRRSV. The inhibition rate (PI) was calculated as follows: PI = [(OD450 of the negative control—OD450 of the test sample)/OD450 of the negative control] × 100%. C Sensitivity evaluation of the ic-ELASA method for PRV detection by performing twofold serial dilutions of PRV viral solution